Gradient-free determination of isoelectric points of proteins on chip-Reference-Cited by-同舟云学术

Gradient-free determination of isoelectric points of proteins on chip

Published:2017 Issue:34 Volume:19 Page:23060-23067
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Łapińska Urszula¹²³⁴,Saar Kadi L.¹²³⁴^ORCID,Yates Emma V.¹²³⁴,Herling Therese W.¹²³⁴,Müller Thomas¹²³⁴⁵,Challa Pavan K.¹²³⁴,Dobson Christopher M.¹²³⁴,Knowles Tuomas P. J.¹²³⁴⁶^ORCID

Affiliation:

1. Department of Chemistry

2. University of Cambridge

3. Cambridge CB2 1EW

4. UK

5. Fluidic Analytics

6. Department of Physics

Abstract

We have developed a method for measuring protein isoelectric points on a microfluidic chip in which gradient changes in time rather then in space.

Funder

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2017/CP/C7CP01503H

Reference71 articles.

1. Determination of the isoelectric point of proteins by capillary isoelectric focusing

2. Generation of a miniaturized free-flow electrophoresis chip based on a multi-lamination technique—isoelectric focusing of proteins and a single-stranded DNA fragment

3. Isoelectric point electrophoresis: a new technique for protein purification

4. R. K. Scopes , Protein Purification Principles and Practice, Springer, Bundoora, Australia, 2nd edn, 1987

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