A dual identification strategy based on padlock ligation and CRISPR/Cas14a for highly specific detection of BRAF V600E mutation in clinical samples-Reference-Cited by-同舟云学术

A dual identification strategy based on padlock ligation and CRISPR/Cas14a for highly specific detection of BRAF V600E mutation in clinical samples

Published:2022 Issue:19 Volume:14 Page:1913-1921
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Shi Weicheng¹,Gong Yao¹,Zhang Decai¹,Yang Tiantian¹,Yi Ming¹,Tan Jingyi¹,Ding Shijia²^ORCID,Cheng Wei¹^ORCID

Affiliation:

1. The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China

2. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China

Abstract

A dual identification strategy based on ligase chain reactions and CRISPR/Cas14a for highly specific SNV detection.

Funder

National Natural Science Foundation of China

Science Fund for Distinguished Young Scholars of Chongqing

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AY/D2AY00319H

Reference38 articles.

1. Mutations of the BRAF gene in human cancer

2. BRAF Mutation Predicts a Poorer Clinical Prognosis for Papillary Thyroid Cancer

3. TheBRAFV600E Mutation Is an Independent, Poor Prognostic Factor for the Outcome of Patients with Low-Risk Intrathyroid Papillary Thyroid Carcinoma: Single-Institution Results from a Large Cohort Study

4. Clinical Implication of Hot SpotBRAFMutation, V599E, in Papillary Thyroid Cancers

5. BRAF mutations in non-Hodgkin's lymphoma

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