A rapid variant-tolerant reverse transcription loop-mediated isothermal amplification assay for the point of care detection of HIV-1

Author:

Li Yingxue123,Chen Xin4,Zhao Yongjuan1,Wan Zhenzhou5,Zeng Yi1,Ma Yingying1,Zhou Lianqun23ORCID,Xu Gaolian6ORCID,Reboud Julien7ORCID,Cooper Jonathan M.7ORCID,Zhang Chiyu1ORCID

Affiliation:

1. Shanghai Clinical Research Center for Infectious Disease (HIV/AIDS), Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China

2. CAS key Laboratory of Bio-medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

3. School of Biomedical Engineering, University of Science and Technology of China, Hefei 260026, China

4. Department of Pathogenic Biology, School of Basic Medical Sciences, Gannan Medical University, Ganzhou 341000, China

5. Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou 225300, China

6. Nano Biomedical Research Centre, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China

7. Division of Biomedical Engineering, University of Glasgow, G12 8LT Glasgow, UK

Abstract

Human immunodeficiency virus (HIV) rapid variant mutations challenges diagnostic assays. Our variant-tolerant isothermal nucleic acid based amplification technique enables reliable detection at the point-of-care.

Funder

National Science and Technology Major Project

Engineering and Physical Sciences Research Council

Scottish Funding Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference30 articles.

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2. UNAIDS: 90-90-90: An Ambitious Treatment Target to Help End the AIDS Epidemic 2017. Available from: https://www.unaids.org/sites/default/files/media_asset/90-90-90_en.pdf

3. Nucleic acid testing and molecular characterization of HIV infections

4. PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings

5. The origin and diversity of the HIV-1 pandemic

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