Improving DNA aptamers against a heart failure protein biomarker using structure-guided random mutation approaches for colourimetric biosensor development-Reference-Cited by-同舟云学术

Improving DNA aptamers against a heart failure protein biomarker using structure-guided random mutation approaches for colourimetric biosensor development

Published:2024 Issue: Volume: Page:
ISSN:2058-9689
Container-title:Molecular Systems Design & Engineering
language:en
Short-container-title:Mol. Syst. Des. Eng.

Author:

Marcius Donny¹,Ropii Bejo²³,Safitri Diah Ayu⁴,Ulum Mokhamad Fakhrul⁵,Nugrahapraja Husna¹,Anshori Isa²⁶⁷^ORCID

Affiliation:

1. School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesa No. 10, Bandung 40132, Indonesia

2. School of Electrical Engineering and Informatics, Institut Teknologi Bandung, Jl. Ganesa No. 10, Bandung 40132, Indonesia

3. Preanger Institute for Biomedical Engineering, Science, and Technology, Indonesia

4. Biomedical R&D Department, PT Hana Master Jaya, Jl. Cibingbin No. 99, Laksanamekar, Kabupaten Bandung Barat 40553, Indonesia

5. School of Veterinary Medicine and Biomedical Sciences, IPB University (Bogor Agricultural University), Jl. Agathis Kampus IPB Dramaga, Bogor 16680, Indonesia

6. Research Center of Nanoscience and Nanotechnology, Bandung Institute of Technology, Bandung 40132, Indonesia

7. Center of Artificial Intelligence, Bandung Institute of Technology, Bandung 40132, Indonesia

Abstract

The optimisation strategy successfully enhanced the DNA aptamer binding capability to its target. The docking, molecular dynamics, and gold nanoparticle aggregation assays showed the performance improvement of optimised aptamer as a bioreceptor.

Funder

Institut Teknologi Bandung

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/ME/D4ME00073K

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