Recombinant supercharged polypeptides for safe and efficient heparin neutralization

Author:

Tu Jianfei1,Liu Qing2,You Shengye2,Meng Zhuojun2,Fang Shiji1,Yu Binhong2,Chen Xumin3,Zhou Yu45,Zeng Lulu2,Herrmann Andreas45ORCID,Chen Gang67,Shen Jianliang2,Zheng Lifei2ORCID,Ji Jiansong1

Affiliation:

1. Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, Imaging Diagnostic and Interventional Minimally Invasive Institute, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000, Zhejiang, China

2. Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, Zhejiang, China

3. Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China

4. DWI - Leibniz Institute for Interactive Materials, Forckenbeckstr 50, 52056 Aachen, Germany

5. Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, Germany

6. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

7. Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

Abstract

Recombinant supercharged unfolded polypeptides display excellent heparin-neutralizing performance and biocompatibilityin vitroandin vivo, showing good potential to replace protamine sulfate, the only clinically licensed antidote for heparin.

Funder

Wenzhou Institute of Biomaterials and Engineering

Natural Science Foundation of Zhejiang Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

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