An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR-Reference-Cited by-同舟云学术

An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR

Published:2014 Issue:19 Volume:14 Page:3739-3749
ISSN:1473-0197
Container-title:Lab Chip
language:en
Short-container-title:Lab Chip

Author:

Rival A.¹²³⁴¹,Jary D.¹⁵⁶⁷⁴,Delattre C.¹⁵⁶⁷⁴,Fouillet Y.¹⁵⁶⁷⁴,Castellan G.¹⁵⁶⁷⁴,Bellemin-Comte A.¹⁵⁶⁷⁴,Gidrol X.¹²³⁴⁸

Affiliation:

1. CEA

2. IRTSV

3. Laboratoire de Biologie à Grande Echelle

4. F-38054 Grenoble Cedex 9, France

5. LETI

6. MINATEC Campus

7. Microtechnologies for Biology and Healthcare Department

8. INSERM

Abstract

A compact EWOD digital microfluidic chip enables single cell manipulation, sample preparation using magnetic beads and gene expression analysis by qRT-PCR.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2014/LC/C4LC00592A

Reference39 articles.

1. Non-genetic heterogeneity of cells in development: more than just noise

2. Single cell resolution in regulation of gene expression

3. Dynamics of single‐cell gene expression

4. Microfluidic Single-Cell mRNA Isolation and Analysis

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