Versatile method for AFM-tip functionalization with biomolecules: fishing a ligand by means of an in situ click reaction-Reference-Cited by-同舟云学术

Versatile method for AFM-tip functionalization with biomolecules: fishing a ligand by means of an in situ click reaction

Published:2015 Issue:15 Volume:7 Page:6599-6606
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Kumar Rakesh¹²³,Ramakrishna Shivaprakash N.⁴⁵²³,Naik Vikrant V.⁴⁵²³,Chu Zonglin¹²³,Drew Michael E.¹²³,Spencer Nicholas D.⁴⁵²³,Yamakoshi Yoko¹²³

Affiliation:

1. Laboratorium für Organische Chemie

2. ETH Zürich

3. CH-8093 Zürich, Switzerland

4. Laboratory for Surface Science and Technology

5. Department of Materials

Abstract

A method for AFM-tip functionalization is described using click chemistry between pre-immobilized tripod–acetylene on the tip and azide-connected biomolecules. It has been successfully applied to chemical force spectroscopy.

Funder

Schweizerische Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2015/NR/C5NR01495F

Reference54 articles.

1. Functional Group Imaging by Chemical Force Microscopy

2. Chemical Force Microscopy: Exploiting Chemically-Modified Tips To Quantify Adhesion, Friction, and Functional Group Distributions in Molecular Assemblies

3. Force Titrations and Ionization State Sensitive Imaging of Functional Groups in Aqueous Solutions by Chemical Force Microscopy

4. High-resolution atomic force microscopy and spectroscopy of native membrane proteins

5. Stretching single polysaccharides and proteins using atomic force microscopy

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