Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases
Author:
Affiliation:
1. Deakin University
2. School of Life and Environmental Sciences
3. Centre for Chemistry and Biotechnology
4. Geelong
5. Australia
Abstract
Solvent incompatibility is a limiting factor when implementing 2D-HPLC. A programmed temperature gradient in the first dimension was developed to overcome this limitation by allowing an isocratic separation while maintaining selectivity.
Funder
Australian Research Council
Publisher
Royal Society of Chemistry (RSC)
Subject
General Engineering,General Chemical Engineering,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2016/AY/C5AY02528A
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