Characterization of phenolic acids binding to thrombin using frontal affinity chromatography and molecular docking-Reference-Cited by-同舟云学术

Characterization of phenolic acids binding to thrombin using frontal affinity chromatography and molecular docking

Published:2017 Issue:35 Volume:9 Page:5174-5180
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Yang Yu-Xiu¹²³⁴,Li Su-Ying⁵⁶⁷⁴,Zhang Qian¹²³⁴,Chen Hua¹²³⁴,Xia Zhi-Ning¹²³⁴,Yang Feng-Qing¹²³⁴^ORCID

Affiliation:

1. School of Chemistry and Chemical Engineering

2. Chongqing University

3. Chongqing 401331

4. PR China

5. Department of Pharmacy

6. Henan Medical College

7. Zhengzhou 451191

Abstract

The thrombin was simply immobilized by IAM chromatography column, and the binding parameters for phenolic acids binding to thrombin were determined by frontal affinity chromatography. The Autodock software was adopted to study the binding model between phenolic acids and thrombin.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AY/C7AY01433C

Reference36 articles.

1. Evidence That Both Exosites on Thrombin Participate in Its High Affinity Interaction with Fibrin

2. Protease-activated receptors in hemostasis, thrombosis and vascular biology

3. Tissue thrombin is associated with the pathogenesis of dilated cardiomyopathy