Bottom-up mass spectrometric sequencing of microRNA-Reference-Cited by-同舟云学术

Bottom-up mass spectrometric sequencing of microRNA

Published:2014 Issue:21 Volume:6 Page:8829-8839
ISSN:1759-9660
Container-title:Anal. Methods
language:en
Short-container-title:Anal. Methods

Author:

Wambua Dickson M.¹²³,Ubukata Masaaki⁴⁵,Dane John⁶⁷³,Cody Robert B.⁴⁵,Chiu Norman H. L.¹²³⁶⁷

Affiliation:

1. Department of Chemistry and Biochemistry

2. University of North Carolina at Greensboro

3. Greensboro, USA

4. JEOL USA, Inc.

5. Peabody, USA

6. Department of Nanoscience

7. Joint School of Nanoscience and Nanoengineering

Abstract

By using the acidity of MALDI matrix at high temperature, sequencing ladders of miRNA were generated. The accurate monoisotopic mass of each RNA fragment was measured by MALDI SpiralTOF. Analysis of nucleotide identity and complementary sequencing ladders allowed 100% sequence coverage and accuracy to be achieved.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AY/C4AY01519C

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