Transglutaminase-mediated proximity labeling of a specific Lys residue in a native IgG antibody

Author:

Nishioka Riko1ORCID,Iida Ryuya1,Minamihata Kosuke1,Sato Ryo1,Kimura Michio1,Kamiya Noriho12ORCID

Affiliation:

1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan

2. Division of Biotechnology, Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan

Abstract

The fusion protein of an engineered microbial transglutaminase and protein G variant enabled site-specific and tag-free labeling of Lys65 of a native IgG with a Gln-donor peptidyl substrate.

Funder

Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

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