New cellulose–silica composite IMAC/C18 for the selective enrichment of phosphorylated molecules and the improved recovery of hydrophilic species
Author:
Affiliation:
1. Division of Analytical Chemistry
2. Institute of Chemical Sciences
3. Bahauddin Zakariya University
4. Multan 60800
5. Pakistan
Abstract
A new methodology is reported for the synthesis of a hydrophilic cellulose–silica composite, followed by derivatization for the selective enrichment of phosphopeptides and use as a desalting material prior to mass spectrometric analysis.
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemical Engineering,General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2015/RA/C4RA10254A
Reference31 articles.
1. A Highly Efficient Multifunctional Tandem Affinity Purification Approach Applicable to Diverse Organisms
2. Facile Fabrication of Hydrophilic Nanofibrous Membranes with an Immobilized Metal–Chelate Affinity Complex for Selective Protein Separation
3. Enhanced Detection of Multiply Phosphorylated Peptides and Identification of Their Sites of Modification
4. Biomarker Discovery in Serum/Plasma Using Surface Enhanced Laser Desorption Ionization Time of Flight (SELDI–TOF) Mass Spectrometry
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