Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes-Reference-Cited by-同舟云学术

Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes

Published:2021 Issue:4 Volume:2 Page:1291-1295
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Gavins Georgina C.¹²³⁴,Gröger Katharina¹²³⁴,Reimann Marc¹²³⁴^ORCID,Bartoschek Michael D.⁵⁶⁷⁴^ORCID,Bultmann Sebastian⁵⁶⁷⁴^ORCID,Seitz Oliver¹²³⁴^ORCID

Affiliation:

1. Institut für Chemie

2. Humboldt-Universität zu Berlin Brook-Taylor-Strasse 2

3. Berlin 12489

4. Germany

5. Center for Molecular Biosystems (BioSysM)

6. Faculty of Biology

7. Ludwig-Maximilians-Universität München, Butenandtstr. 1

Abstract

A pair of orthogonal coiled coils templates highly specific live cell bioconjugation of two different proteins. PNA tagging and hybridisation with fluorophore–DNA reporters enables rapid dual receptor internalisation analysis of EGFR and ErbB2.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2021/CB/D1CB00126D

Reference36 articles.

1. The Fluorescent Toolbox for Assessing Protein Location and Function

2. Chemical Tags for Labeling Proteins Inside Living Cells

3. Imaging and manipulating proteins in live cells through covalent labeling

4. Selective chemical labeling of proteins

5. Peptide-tags for site-specific protein labelling in vitro and in vivo

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