Preparative capillary electrophoresis (CE) fractionation of protein digests improves protein and peptide identification in bottom-up proteomics-Reference-Cited by-同舟云学术

Preparative capillary electrophoresis (CE) fractionation of protein digests improves protein and peptide identification in bottom-up proteomics

Published:2022 Issue:11 Volume:14 Page:1103-1110
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Weaver Simon D.¹²^ORCID,Schuster-Little Naviya²^ORCID,Whelan Rebecca J.²^ORCID

Affiliation:

1. Integrated Biomedical Sciences Graduate Program, University of Notre Dame, Notre Dame, IN, USA

2. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA

Abstract

Fractionating samples with preparative capillary electrophoresis before liquid chromatography-mass spectrometry enables greater coverage of a cancer biomarker and deeper sequencing of a complex proteome.

Funder

University of Notre Dame

National Institute of General Medical Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AY/D1AY02145A

Reference64 articles.

1. A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

2. Protein Analysis by Shotgun/Bottom-up Proteomics

3. Comparison of CE-MS/MS and LC-MS/MS sequencing demonstrates significant complementarity in natural peptide identification in human urine

4. Multidimensional separation of tryptic peptides from human serum proteins using reversed-phase, strong cation exchange, weak anion exchange, and fused-core fluorinated stationary phases

5. Some possibilities of an analysis of complex samples by a mass spectrometry with a sample pretreatment by an offline coupled preparative capillary isotachophoresis

Cited by 11 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Integrated MICROFASP Method with CZE-Based Fractionation Technique and NanoRPLC-ESI-MS/MS for a Comprehensive Proteomics Analysis of a Submicrogram Sample;Journal of Proteome Research;2024-07-16

2. Capillary Electrophoresis: A Three-Year Literature Review;Analytical Chemistry;2024-04-10

3. Fit for Purpose Approach To Evaluate Detection of Amino Acid Substitutions in Shotgun Proteomics;Journal of Proteome Research;2024-03-13

4. A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing;Cancer Research Communications;2024-01-31

5. An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays;Methods in Molecular Biology;2024