β-Carbonyl sulfonium enables cysteine-specific bioconjugation for activity-based protein profiling in live cells-Reference-Cited by-同舟云学术

β-Carbonyl sulfonium enables cysteine-specific bioconjugation for activity-based protein profiling in live cells

Published:2024 Issue:27 Volume:60 Page:3725-3728
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Wan Chuan¹,Yang Dongyan²,Guo Xiaochun³,Zhang Tuanjie⁴,Ruan Zhijun⁴,Dai Chuan⁴,Xing Yun³,Yin Feng³⁴,Wang Rui⁴^ORCID,Li Zigang³⁴^ORCID

Affiliation:

1. College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, 518118, P. R. China

2. Innovative Institute for Plant Health, College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, P. R. China

3. State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 518055, P. R. China

4. Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen, 518055, P. R. China

Abstract

Small electrophilic molecules have previously been reported to monitor different reactivities or covalent ligand abilities of nucleophilic residues in proteins. Herein, we develop β-carbonyl sulfonium compounds to selectively modify cysteines in peptides and proteins in live cells.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Basic and Applied Basic Research Foundation of Guangdong Province

Science, Technology and Innovation Commission of Shenzhen Municipality

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CC/D4CC00295D

Reference36 articles.

1. A Proximity-Triggered Strategy toward Transferable Proteolysis Targeting Chimeras

2. Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells

3. Proteome-wide covalent ligand discovery in native biological systems

4. A proteome-wide atlas of lysine-reactive chemistry

5. Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome