Electrostatic microfiltration (EM) enriches and recovers viable microorganisms at low-abundance in large-volume samples and enhances downstream detection

Author:

Liu Yaoping12ORCID,Raymond Joshua J.2ORCID,Wu Xiaolin2ORCID,Chua Patrina Wei Lin1,Ling Sharon Yan Han1,Chan Chia Ching1,Chan Cheryl2,Loh Joanne Xin Yi2,Song Melody Xing Yen3,Ong Matilda Yu Yan3,Ho Peiying1ORCID,Mcbee Megan E.1ORCID,Springs Stacy L.24,Yu Hanry256,Han Jongyoon12478ORCID

Affiliation:

1. AntiMicrobial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART), 138602, Singapore

2. Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, Singapore-MIT Alliance for Research and Technology (SMART), 138602, Singapore

3. School of Life Sciences & Chemical Technology, Ngee Ann Polytechnic, 599489, Singapore

4. Center for Biomedical Innovation, Massachusetts Institute of Technology (MIT), MA 02139, USA

5. Institute of Bioengineering and Bioimaging (IBB), A*STAR, 138632, Singapore

6. Department of physiology and WisDM and Mechanobiology Institute, National University of Singapore, 119077, Singapore

7. Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA

8. Department of Biological Engineering, MIT, Cambridge, MA 02139, USA

Abstract

Electrostatic microfiltration (EM) for enrichment and recovery of low-abundance viable microorganisms from large-volume samples: enhancing downstream detection of pathogens below sampling limits.

Funder

National Research Foundation Singapore

Publisher

Royal Society of Chemistry (RSC)

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