Stability analysis of chemically modified mRNA using micropattern-based single-cell arrays-Reference-Cited by-同舟云学术

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Stability analysis of chemically modified mRNA using micropattern-based single-cell arrays

Published:2015 Issue:17 Volume:15 Page:3561-3571
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Ferizi Mehrije¹²³⁴,Leonhardt Carolin⁵⁶³⁷,Meggle Christian⁵⁶³⁷,Aneja Manish K.⁸⁹¹⁰,Rudolph Carsten⁸⁹¹⁰,Plank Christian¹²³⁴⁸,Rädler Joachim O.⁵⁶³⁷

Affiliation:

1. Institute of Molecular Immunology - Experimental Oncology

2. Technische Universität München

3. Munich

4. 81675 Germany

5. Faculty of Physics and Center for NanoScience

6. Ludwig Maximilian University

7. 80539 Germany

8. Ethris GmbH

9. Planegg

10. 82152 Germany

Abstract

Micro-structured arrays allow highly parallel assessment of single-cell mRNA-mediated dsGFP-expression time courses. Fits to the individual expression kinetics yield mRNA life-time distribution, which are compared for differently stabilized mRNA constructs.

Funder

Seventh Framework Programme

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2015/LC/C5LC00749F

Reference40 articles.

1. mRNA as gene therapeutic: How to control protein expression

2. Current prospects for mRNA gene delivery

3. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice

4. Non-coding RNAs in human disease

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