Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels-Reference-Cited by-同舟云学术

Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels

Published:2020 Issue:23 Volume:145 Page:7724-7735
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Bickner A. N.¹²³,Champion M. M.¹²³^ORCID,Hummon A. B.⁴⁵⁶³^ORCID,Bruening M. L.¹²³⁷⁸^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry University of Notre Dame

2. Notre Dame

3. USA

4. Department of Chemistry and Biochemistry and the Comprehensive Cancer Center

5. The Ohio State University

6. Columbus

7. Department of Chemical and Biomolecular Engineering

8. University of Notre Dame

Abstract

Electroblotting through a trypsin-containing membrane simplifies extraction and digestion of proteins separated by SDS-PAGE. Analysis by LC-MS/MS identifies the extracted proteins.

Funder

National Institutes of Health

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/D0AN01380C

Reference48 articles.

1. Gel-based proteomics in disease research: Is it still valuable?

2. Comparison of bottom-up proteomic approaches for LC-MS analysis of complex proteomes

3. Top-down/Bottom-up Mass Spectrometry Workflow Using Dissolvable Polyacrylamide Gels

4. Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application

5. Gel-Based Proteomics of Clinical Samples Identifies Potential Serological Biomarkers for Early Detection of Colorectal Cancer

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