An integrative temperature-controlled microfluidic system for budding yeast heat shock response analysis at the single-cell level-Reference-Cited by-同舟云学术

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An integrative temperature-controlled microfluidic system for budding yeast heat shock response analysis at the single-cell level

Published:2024 Issue:15 Volume:24 Page:3658-3667
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Hong Jie¹²,He Hao³,Xu Yinjia¹,Wang Shujing¹²,Luo Chunxiong¹²³^ORCID

Affiliation:

1. The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China

2. Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China

3. Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China

Abstract

Three forms of temperature changes (step, gradient, and oscillations) were realized in a novel microfluidic platform. The feasibility of this temperature-controlled platform for studying the protein dynamic behavior of yeast cells was demonstrated.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D4LC00313F

Reference43 articles.

1. Stress-induced growth rate reduction restricts metabolic resource utilization to modulate osmo-adaptation time

2. Rate of environmental change determines stress response specificity

3. Adaptive prediction of environmental changes by microorganisms

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