Heterologous expression of the cryptic <i>mdk</i> gene cluster and structural revision of maduralactomycin A-Reference-Cited by-同舟云学术

Heterologous expression of the cryptic mdk gene cluster and structural revision of maduralactomycin A

Published:2023 Issue:48 Volume:13 Page:34136-34144
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Schwitalla Jan W.¹²^ORCID,Le Ngoc-Thao-Hien³,Um Soohyun⁴,Schalk Felix¹^ORCID,Brönstrup Mark⁵^ORCID,Baunach Martin⁶,Beemelmanns Christine¹²⁷^ORCID

Affiliation:

1. Chemical Biology of Microbe-Host Interactions, Hans-Knöll Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany

2. Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Campus E8, 66123, Saarbrücken, Germany

3. Department of Pharmaceutical Sciences, Natural Products and Food Research and Analysis (NatuRA), University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium

4. College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, 21983, South Korea

5. Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany

6. Institute of Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany

7. Saarland University, 66123, Saarbrücken, Germany

Abstract

After conducting an in silico analysis of the cryptic mdk cluster region and performing transcriptomic studies, an integrative Streptomyces BAC Vector containing the mdk gene sequence was constructed and heterologous expression yielded the angucyclic product seongomycin.

Funder

Deutsche Forschungsgemeinschaft

Ministry of Science and ICT, South Korea

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/RA/D3RA05931F

Reference46 articles.

1. Defense contracts: molecular protection in insect-microbe symbioses

2. Towards the sustainable discovery and development of new antibiotics

3. Ecology and genomics of Actinobacteria: new concepts for natural product discovery

4. Leveraging ecological theory to guide natural product discovery

5. Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Hot off the press;Natural Product Reports;2024