A novel nanogel-based fluorescent probe for ratiometric detection of intracellular pH values

Author:

Cao Lixia1234,Li Xiaoyan1234,Wang Shuangqing1234,Li Shayu1234,Li Yi564,Yang Guoqiang1234

Affiliation:

1. Beijing National Laboratory for Molecular Sciences

2. Key Laboratory of Photochemistry

3. Institute of Chemistry

4. Beijing 100190, China

5. Key Laboratory of Photochemical Conversion and Optoelectronic Materials

6. Technical Institute of Physics and Chemistry

Abstract

A new nanogel indicator (NGI) for ratiometric fluorescent detection of intracellular pH values has been reported. In aqueous solution, the NGI can be used for ratiometric sensing of pH changes with a large hypsochromic shift of 100 nm in the green–red region and reversible. Furthermore, the NGI has been used to calibrate the cytosol pH in living cells.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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