Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR-Reference-Cited by-同舟云学术

Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR

Published:2020 Issue:3 Volume:20 Page:477-489
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Abram Timothy J.¹²³,Cherukury Hemanth⁴⁵²³⁶,Ou Chen-Yin¹²³,Vu Tam⁴⁵²³⁷,Toledano Michael⁴⁵²³,Li Yiyan⁴⁵²³⁸,Grunwald Jonathan T.¹²³,Toosky Melody N.¹²³,Tifrea Delia F.⁹¹⁰²³,Slepenkin Anatoly⁹¹⁰²³,Chong Jonathan⁴⁵²³,Kong Lingshun⁴⁵²³,Del Pozo Domenica Vanessa⁴⁵²³,La Kieu Thai⁴⁵²³,Labanieh Louai⁴⁵²³,Zimak Jan⁴⁵²³,Shen Byron¹²³,Huang Susan S.¹¹¹²²³,Gratton Enrico⁷⁵²³¹³,Peterson Ellena M.⁹¹⁰²³,Zhao Weian⁴⁵²³⁶^ORCID

Affiliation:

1. Velox Biosystems

2. Irvine

3. USA

4. Sue and Bill Gross Stem Cell Research Center

5. University of California, Irvine

6. Department of Pharmaceutical Sciences

7. Department of Biomedical Engineering

8. Department of Physics and Engineering

9. Department of Pathology and Laboratory Medicine

10. University of California

11. Division of Infectious Diseases

12. UCI School of Medicine

13. Laboratory for Fluorescence Dynamics

Abstract

We report a rapid diagnostic platform that integrates novel one-step blood droplet PCR assay and a high throughput droplet counting system to perform bacterial identification and antibiotic susceptibility profiling directly from whole blood.

Funder

National Institute of Allergy and Infectious Diseases

National Institutes of Health

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/C9LC01212E

Reference52 articles.

1. The Alliance for the, Prudent Use of Antibiotics. The cost of antibiotic resistance to U.S. families and the health care system, 2010