Co-factor prosthesis facilitates biosynthesis of azido-pseudaminic acid probes for use as glycosyltransferase reporters

Author:

Keenan Tessa1ORCID,Chidwick Harriet S.1,Best Matthew1,Flack Emily K. P.12,Yates Nicholas D. J.1,Hatton Natasha E.1,Warnes Matthew E.1,Fascione Martin A.1ORCID

Affiliation:

1. Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK

2. Department of Biology, University of York, York, YO10 5DD, UK

Abstract

PseH “co-factor prothesis” strategy with N-acetyl-S-azidocysteamine, in the biosynthesis of N7-azido-functionalised pseudaminic acid, a reporter of pseudaminyltransferase activity.

Funder

UK Research and Innovation

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Reference27 articles.

1. A. L.Lewis , X.Chen , R. L.Schnaar and A.Varki , in Essentials of Glycobiology , ed. A. Varki , R. D. Cummings , J. D. Esko , P. Stanley , G. W. Hart , M. Aebi , D. Mohnen , T. Kinoshita , N. H. Packer , J. H. Prestegard , R. L. Schnaar and P. H. Seeberger , Cold Spring Harbor Laboratory Press , Cold Spring Harbor (NY) , 4th edn, 2022

2. Identification of the Carbohydrate Moieties and Glycosylation Motifs in Campylobacter jejuni Flagellin

3. Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori

4. Sialic acids of a new type from the lipopolysaccharides of Pseudomonas aeruginosa and Shigella boydii

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