Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease

Author:

Lei Sheng12,Liu Zi3,Zhang Qidong1,Li Zezhi14,Li Gaiping5ORCID,Zou Lina5ORCID,Chai Guobi1,Mao Jian16,Ye Baoxian5ORCID,Zhang Jianxun1

Affiliation:

1. Zhengzhou Tobacco Research Institute of China National Tobacco Company, Zhengzhou 450001, P. R. China

2. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, P. R. China

3. Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, P. R. China

4. School of Light Industry, Beijing Technology and Business University, Beijing 100048, P. R. China

5. College of Chemistry, Zhengzhou University, Zhengzhou 450001, P. R. China

6. Beijing Life Science Academy, Beijing, 102299, P. R. China

Abstract

A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was investigated for ultrasensitive detection of miRNA.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Henan Province

Publisher

Royal Society of Chemistry (RSC)

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