A new spectrophotometric assay for measuring pyruvate dehydrogenase complex activity: a comparative evaluation-Reference-Cited by-同舟云学术

A new spectrophotometric assay for measuring pyruvate dehydrogenase complex activity: a comparative evaluation

Published:2014 Issue:16 Volume:6 Page:6381-6388
ISSN:1759-9660
Container-title:Anal. Methods
language:en
Short-container-title:Anal. Methods

Author:

Ke Chen-Juan¹²³⁴,He Ya-Hui⁵⁶,He Hong-Wu⁷⁸⁹¹⁰³,Yang Xu¹²³⁴,Li Rui¹²³⁴,Yuan Junlin¹²³⁴

Affiliation:

1. Huibei Key Laboratory of Genetic Regulation and Integrative Biology

2. School of Life Sciences

3. Central China Normal University

4. Wuhan 430079, China

5. Pingdingshan Center of Bureau of Quality and Technical Supervision Inspection and Test

6. Ping-dingshan 467000, China

7. Key Laboratory of Pesticide and Chemical Biology

8. Ministry of Education

9. Institute of Pesticide Chemistry

10. School of Chemistry

Abstract

We describe a new spectrophotometric assay for measuring pyruvate dehydrogenase complex activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as an electron acceptor.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AY/C4AY00804A

Reference22 articles.

1. Purification of the Pyruvate Dehydrogenase Multienzyme Complex of Zymomonas mobilis and Identification and Sequence Analysis of the Corresponding Genes

2. Studies of O,O-Dimethyl α-(2,4-Dichlorophenoxyacetoxy)ethylphosphonate (HW02) as a New Herbicide. 1. Synthesis and Herbicidal Activity of HW02 and Analogues as Novel Inhibitors of Pyruvate Dehydrogenase Complex

3. Structural Basis for Flip-Flop Action of Thiamin Pyrophosphate-dependent Enzymes Revealed by Human Pyruvate Dehydrogenase

4. α-Keto acid dehydrogenase complexes. XIV. Regulation of the activity of the pyruvate dehydrogenase complex of Escherichia coli

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