Development of a chromophore-solid phase peptide reaction assay (C-SPRA) for assessing skin sensitization in vitro-Reference-Cited by-同舟云学术

Development of a chromophore-solid phase peptide reaction assay (C-SPRA) for assessing skin sensitization in vitro

Published:2020 Issue:9 Volume:145 Page:3211-3216
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Miyazaki Hiroshi¹²³⁴⁵,Hamada Yoshio⁶⁷⁸⁵^ORCID,Takaishi Hikaru⁶⁷⁸⁵,Minamino Yuuki⁶⁷⁸⁵,Ikeda Hidefumi⁹¹⁰¹¹⁵,Mekata Hideaki⁹¹⁰¹¹⁵,Takaishi Masayuki⁹¹⁰¹¹⁵,Yamashita Kunihiko¹²³⁴⁵,Usui Kenji⁶⁷⁸⁵^ORCID

Affiliation:

1. Medical Device Division

2. Innovation and Business Development Headquarters

3. Daicel Corporation

4. Tokyo 108-8230

5. Japan

6. Faculty of Frontiers of Innovative Research in Science and Technology (FIRST)

7. Konan University

8. Kobe 650-0047

9. Product Assurance Division

10. Mandom Corporation

11. Osaka 540-8530

Abstract

We developed an in vitro chromophore-solid phase peptide reaction assay (C-SPRA) using chromophores and immobilized peptides on microbeads. C-SPRA would enable accurate and high-throughput assessments of various chemicals.

Funder

Towa Foundation for Food Science and Research

Nipponham Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/C9AN02514F

Reference39 articles.

1. Development of a murine local lymph node assay for the determination of sensitizing potential

2. Development of a Peptide Reactivity Assay for Screening Contact Allergens

3. Quantification of Chemical Peptide Reactivity for Screening Contact Allergens: A Classification Tree Model Approach

4. Early immune events in the induction of allergic contact dermatitis

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