Nanoparticle-mediated CRISPR/dCas9a activation of multiple transcription factors to engineer insulin-producing cells-Reference-Cited by-同舟云学术

Nanoparticle-mediated CRISPR/dCas9a activation of multiple transcription factors to engineer insulin-producing cells

Published:2023 Issue:9 Volume:11 Page:1866-1870
ISSN:2050-750X
Container-title:Journal of Materials Chemistry B
language:en
Short-container-title:J. Mater. Chem. B

Author:

Lee Mei-Hwa¹,Thomas James L.²,Lin Chien-Yu³,Li Yi-Chen Ethan⁴,Lin Hung-Yin³^ORCID

Affiliation:

1. Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan

2. Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA

3. Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan

4. Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan

Abstract

Four gRNAs were synthesized and used to form ribonucleoproteins (RNPs) with tracRNA and dCas9-VPR, and were then immobilized on magnetic peptide-imprinted chitosan nanoparticles, which enhanced transfection for production of insulin.

Funder

Ministry of Science and Technology, Taiwan

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering,General Chemistry,General Medicine

Link

http://pubs.rsc.org/en/content/articlepdf/2023/TB/D2TB02431D

Reference24 articles.

1. Insulin/Glucose-Responsive Cells Derived from Induced Pluripotent Stem Cells: Disease Modeling and Treatment of Diabetes

2. The potential and challenges of alternative sources of β cells for the cure of type 1 diabetes

3. Efficiency of Stem Cell (SC) Differentiation into Insulin-Producing Cells for Treating Diabetes: a Systematic Review

4. The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation

5. Controlled clustering enhances PDX1 and NKX6.1 expression in pancreatic endoderm cells derived from pluripotent stem cells

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