Handheld real-time PCR device-Reference-Cited by-同舟云学术

Handheld real-time PCR device

Published:2016 Issue:3 Volume:16 Page:586-592
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Ahrberg Christian D.¹²³⁴,Ilic Bojan Robert⁵⁶⁷⁸,Manz Andreas¹²³⁴,Neužil Pavel¹²³⁴⁹

Affiliation:

1. KIST-Europe

2. Microfluidics Group

3. 66111 Saarbrücken

4. Germany

5. National Institute of Standard and Technology (NIST)

6. Center for Nanoscale Science and Technology

7. Gaithersburg

8. USA

9. Brno University of Technology (BUT)

Abstract

World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use in small clinics and point-of-care applications.

Funder

Central European Institute of Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C5LC01415H

Reference24 articles.

1. Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

2. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

3. PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings