Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes-Reference-Cited by-同舟云学术

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Published:2019 Issue:39 Volume:10 Page:8995-9000
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Lanyon-Hogg Thomas¹²³⁴^ORCID,Ritzefeld Markus¹²³⁴^ORCID,Sefer Lea⁵⁶⁷⁸⁴,Bickel Jasmine K.¹²³⁴^ORCID,Rudolf Amalie F.⁵⁶⁷⁸⁴,Panyain Nattawadee¹²³⁴^ORCID,Bineva-Todd Ganka⁹¹⁰⁴,Ocasio Cory A.⁹¹⁰⁴,O'Reilly Nicola⁹¹⁰⁴^ORCID,Siebold Christian⁵⁶⁷⁸⁴,Magee Anthony I.¹¹¹²²³⁴^ORCID,Tate Edward W.¹²³⁴^ORCID

Affiliation:

1. Department of Chemistry

2. Imperial College London

3. London

4. UK

5. Division of Structural Biology

6. Wellcome Centre for Human Genetics

7. University of Oxford

8. Oxford

9. The Francis Crick Institute

10. London NW1 1AT

11. Molecular Medicine Section

12. National Heart & Lung Institute

Abstract

A highly accurate and versatile fluorescence polarisation assay for any enzyme adding or removing lipid posttranslational modifications, with the potential to accelerate drug discovery against these targets.

Funder

FP7 People: Marie-Curie Actions

Medical Research Council

Wellcome Trust

Cancer Research UK

Engineering and Physical Sciences Research Council

H2020 European Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

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