Simultaneous detection of CaMV35S and T-nos utilizing CRISPR/Cas12a and Cas13a with multiplex-PCR (MPT-Cas12a/13a)-Reference-Cited by-同舟云学术

Simultaneous detection of CaMV35S and T-nos utilizing CRISPR/Cas12a and Cas13a with multiplex-PCR (MPT-Cas12a/13a)

Published:2022 Issue:43 Volume:58 Page:6328-6331
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Cao Gaihua¹,Dong Jiangbo¹,Chen Xiaolong¹^ORCID,Lu Peng²,Xiong Yifan¹,Peng Lan³,Li Jiawei¹²,Huo Danqun¹⁴,Hou Changjun¹^ORCID

Affiliation:

1. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, P. R. China

2. Chongqing University Three Gorges Hospital, Chongqing, 404000, P. R. China

3. Chongqing Medical and Pharmaceutical College Basic Department, Chongqing, 401331, P. R. China

4. Chongqing Key laboratory of Bio-perception & intelligent information Processing, School of microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, P. R. China

Abstract

MPT-Cas12a/13a for a specific and simultaneous assay of CaMV35S and T-nos based on CRISPR/Cas12a and Cas13a with different fluorescence signals.

Funder

Chongqing Science and Technology Commission

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D2CC01300B

Reference30 articles.

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3. Recombinase Polymerase Amplification (RPA) of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops

4. End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor

5. Ultrafast, universal and visual screening of dual genetically modified elements based on dual super PCR and a lateral flow biosensor

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