Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging

Author:

Beal David M1ORCID,Liang Mingzhi1,Brown Ian1,Budge James D1,Burrows Emily R2,Howland Kevin1,Lee Phoebe1,Martin Sarah3,Morrell Andrew1,Nemoto-Smith Emi4,Roobol Joanne1,Stanley Maria1,Smales C Mark15,Warren Martin J167

Affiliation:

1. School of Biosciences, Division of Natural Sciences, University of Kent, Canterbury, UK

2. GSK, Stevenage, UK

3. Catapult: Cell and Gene Therapy, London, UK

4. Charles River, Cambridge, UK

5. National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co, Dublin, A94 X099, Ireland

6. Quadram Institute Bioscience, Norwich, UK

7. Norwich Medical School, University of East Anglia, Norwich, UK

Abstract

A post-translational strategy for the production of SpyTagged molecules suitable for use in Spy based conjugations is described.

Funder

Wellcome Trust

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Chemistry (miscellaneous)

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