Phage-mediated double-nanobody sandwich immunoassay for detecting alpha fetal protein in human serum

Author:

Su Benchao12345,Wang Yidan12345,Pei Hua6784,Sun Zhichang12345,Cao Hongmei12345,Zhang Chenghui12345,Chen Qi12345,Liu Xing12345ORCID

Affiliation:

1. College of Food Science and Engineering

2. Hainan University

3. Haikou 570228

4. China

5. Key Laboratory of Food Nutrition and Functional Food of Hainan Province

6. Department of Clinical Laboratory

7. The Second Affiliated Hospital of Hainan Medical University

8. Haikou 570311

Abstract

A double-antibody sandwich ELISA using a nanobody/phage-displayed nanobody pair for sensitive detection of alpha fetal protein in human serum.

Funder

Hainan University

Natural Science Foundation of Hainan Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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