Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo

Author:

Zakaszewski Daniel12ORCID,Koziej Lukasz1,Pankowski Jędrzej13ORCID,Malolan V. Vishal12,Gämperli Nina4ORCID,Heddle Jonathan G.1ORCID,Hilvert Donald4ORCID,Azuma Yusuke14ORCID

Affiliation:

1. Malopolska Centre of Biotechnology (MCB), Jagiellonian University, Gronostajowa 7A, 30387 Krakow, Poland

2. Doctoral School of Exact and Natural Sciences, Jagiellonian University, Prof. S. Łojasiewicza 11, 30348 Krakow, Poland

3. Faculty of Biochemistry, Biophysics, sand Biotechnology, Jagiellonian University, Gronostajowa 7, 30387 Krakow, Poland

4. Laboratory of Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland

Abstract

This study provides proof of charge complementarity-based inclusion complex formation between an engineered protein nanocage and an otherwise degradation-prone cargo protein in live bacterial cells.

Funder

Narodowe Centrum Nauki

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering,General Chemistry,General Medicine

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