Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method-Reference-Cited by-同舟云学术

Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

Published:2017 Issue:43 Volume:53 Page:5846-5849
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Zhang Kai¹²³⁴⁵^ORCID,Wang Ke¹²³⁴⁵,Zhu Xue¹²³⁴⁵,Xie Minhao¹²³⁴⁵

Affiliation:

1. Key Laboratory of Nuclear Medicine

2. Ministry of Health

3. Jiangsu Key Laboratory of Molecular Nuclear Medicine

4. Jiangsu Institute of Nuclear Medicine

5. Wuxi

Abstract

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity. The detection limits of MITF and NF-κB p65 are 0.23 pM and 0.496 pM, respectively.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2017/CC/C7CC02231J

Reference44 articles.

1. Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity

2. Quantification of Transcription Factor Binding in Cell Extracts Using an Electrochemical, Structure-Switching Biosensor

3. Sensitive Detection of Transcription Factors by Isothermal Exponential Amplification-Based Colorimetric Assay

4. Sensitive Detection of Transcription Factors Using Near-Infrared Fluorescent Solid-Phase Rolling Circle Amplification

5. Sensitive detection of transcription factors in cell nuclear extracts by using a molecular beacons based amplification strategy

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