Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231-Reference-Cited by-同舟云学术

Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231

Published:2017 Issue:11 Volume:142 Page:2038-2049
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Kapoore Rahul Vijay¹²³⁴⁵^ORCID,Coyle Rachael¹²³⁴⁵,Staton Carolyn A.⁶⁷⁸⁴⁵,Brown Nicola J.⁶⁷⁸⁴⁵^ORCID,Vaidyanathan Seetharaman¹²³⁴⁵^ORCID

Affiliation:

1. ChELSI Institute

2. Advanced Biomanufacturing Centre

3. Department of Chemical and Biological Engineering

4. The University of Sheffield

5. Sheffield

6. Department of Oncology and Metabolism

7. Faculty of Dentistry

8. Medicine and Health

Abstract

1 step of PBS wash followed by quenching with 60% methanol supplemented with 70 mM HEPES results in minimal metabolite leakage.

Funder

Engineering and Physical Sciences Research Council

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AN/C7AN00207F

Reference36 articles.

1. Cell culture metabolomics: applications and future directions

2. Metabolomics as a complementary tool in cell culture

3. Comparison of extraction conditions and normalization approaches for cellular metabolomics of adherent growing cells with GC-MS

4. Understanding the biology of triple-negative breast cancer

5. Triple-negative breast cancer—current status and future directions

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