Synthetic engineering of a new biocatalyst encapsulating [NiFe]-hydrogenases for enhanced hydrogen production

Author:

Jiang Qiuyao1,Li Tianpei12,Yang Jing13,Aitchison Catherine M.3ORCID,Huang Jiafeng1,Chen Yu1,Huang Fang1,Wang Qiang2,Cooper Andrew I.3ORCID,Liu Lu-Ning14ORCID

Affiliation:

1. Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK

2. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475004, China

3. Materials Innovation Factory and Department of Chemistry, University of Liverpool, Liverpool L7 3NY, UK

4. College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China

Abstract

By incorporating [NiFe]-hydrogenases into a proteinaceous carboxysome shell, we generate a novel biocatalyst that has improved production of clean hydrogen, oxygen tolerance, and thermostability, highlighting its great potential in biotechnological applications.

Funder

Biotechnology and Biological Sciences Research Council

Leverhulme Trust

Royal Society

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering,General Chemistry,General Medicine

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