Simultaneous monitoring of HIV viral load and screening of SARS-CoV-2 employing a low-cost RT-qPCR test workflow

Author:

Gulati Gaurav K.1ORCID,Panpradist Nuttada12ORCID,Stewart Samuel W. A.13,Beck Ingrid A.3,Boyce Ceejay34,Oreskovic Amy K.1ORCID,García-Morales Claudia5ORCID,Avila-Ríos Santiago5ORCID,Han Peter D.67,Reyes-Terán Gustavo8,Starita Lea M.67ORCID,Frenkel Lisa M.39,Lutz Barry R.17ORCID,Lai James J.1ORCID

Affiliation:

1. Department of Bioengineering, University of Washington, Seattle, Washington, USA

2. Global Health of Women, Adolescents, and Children (Global WACh), School of Public Health, University of Washington, Seattle, Washington, USA

3. Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA

4. Department of Global Health, University of Washington, Seattle, Washington, USA

5. Centre for Research in Infectious Diseases of the National Institute of Respiratory Diseases (CIENI/INER), Mexico City, Mexico

6. Department of Genome Sciences, Seattle, WA, USA

7. Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

8. Coordination of the Mexican National Institutes of Health and High Specialty Hospitals, Mexico City, Mexico

9. Departments of Medicine, Pediatrics, Laboratory Medicine and Pathology, Global Health and Medicine, University of Washington, Seattle, Washington, USA

Abstract

This new workflow enables co-extraction of HIV and SARS-CoV2 RNAs from clinical pooled plasma/nasal secretion samples that allows sensitive detection of SARS-CoV-2 and HIV infections in the patients-living with HIV.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference45 articles.

1. Tuberculosis and HIV responses threatened by COVID-19

2. HIV.gov, Coronavirus (COVID-19) and People with HIV, https://www.hiv.gov/hiv-basics/staying-in-hiv-care/other-related-health-issues/coronavirus-covid-19

3. World Health Organization, WHO: access to HIV medicines severely impacted by COVID-19 as AIDS response stalls, https://www.who.int/news/item/06-07-2020-who-access-to-hiv-medicines-severely-impacted-by-covid-19-as-aids-response-stalls

4. The Burden of COVID-19 in People Living with HIV: A Syndemic Perspective

5. Impact of coronavirus pandemic and containment measures on HIV diagnosis

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