Site-specific RNA modification via initiation of in vitro transcription reactions with m6A and isomorphic emissive adenosine analogs-Reference-Cited by-同舟云学术

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Site-specific RNA modification via initiation of in vitro transcription reactions with m⁶A and isomorphic emissive adenosine analogs

Published:2024 Issue:5 Volume:5 Page:454-458
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Cong Deyuan¹,Steinbuch Kfir B.¹^ORCID,Koyama Ryosuke¹,Lam Tyler V.¹,Lam Jamie Y.¹,Tor Yitzhak¹^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA

Abstract

Transcription reactions with excess adenosine surrogates leads to 5′-end modified transcripts, which can be ligated to diverse RNAs. By modifying a key position in a MazF RNA substrate, insight into the folding and recognition features is gained.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CB/D4CB00045E

Reference31 articles.

1. The role of general initiation factors in transcription by RNA polymerase II

2. Promoter-specific activation of RNA polymerase II transcription by Sp1

3. The mechanism of RNA 5′ capping with NAD+, NADH and desphospho-CoA

4. Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

5. Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcription

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