Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Author:

Yeap Cheryl S.Y.123ORCID,Chaibun Thanyarat13,Lee Su Yin4ORCID,Zhao Bin56,Jan Yuan57ORCID,La-o-vorakiat Chan3ORCID,Surareungchai Werasak38,Song Shiping5,Lertanantawong Benchaporn13ORCID

Affiliation:

1. Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand

2. Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 1402/2, 461 17 Liberec, Czech Republic

3. Pilot Plant and Development Training Institute, Nanoscience and Nanotechnology Research Cluster, King Mongkut's University of Technology Thonburi (KMUTT), Bang Khun Thian Campus, Bangkok 10150, Thailand

4. Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) and Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah, 08100, Malaysia

5. Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China

6. Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

7. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

8. School of Bioresources & Technology, King Mongkut's University of Technology Thonburi, Thailand

Abstract

Multiplex target hybridization and electrochemical detection of 3 enteropathogens (ST: S. typhi; SF: S. flexneri; and VC: V. cholerae).

Funder

National Nanotechnology Center

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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