Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC-Reference-Cited by-同舟云学术

Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC

Published:2019 Issue:16 Volume:144 Page:4985-4994
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Nwokeoji Alison O.¹²³⁴,Kumar Sandip⁵⁶³⁴,Kilby Peter M.⁷⁸⁹⁴,Portwood David E.⁷⁸⁹⁴,Hobbs Jamie K.⁵⁶³⁴,Dickman Mark J.¹²³⁴^ORCID

Affiliation:

1. Department of Chemical and Biological Engineering

2. Mappin Street

3. University of Sheffield

4. UK

5. Department of Physics and Astronomy

6. Hounsfield Road

7. Syngenta

8. Jealott's Hill International Research Centre

9. Bracknell

Abstract

Atomic force microscopy (AFM) in conjunction with ion-pair reverse-phase high performance liquid chromatography (IP-RP-HPLC) provides novel insight into dsRNA for RNAi applications.

Funder

Biotechnology and Biological Sciences Research Council

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AN/C9AN00954J

Reference36 articles.

1. RNAi and double-strand RNA

2. RNAi for Insect Control: Current Perspective and Future Challenges

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5. Improvement of Pest Resistance in Transgenic Tobacco Plants Expressing dsRNA of an Insect-Associated Gene EcR

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