A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction-Reference-Cited by-同舟云学术

A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction

Published:2019 Issue:4 Volume:144 Page:1187-1196
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhou Hongyan¹²³⁴,Tong Chunyi¹²³⁴^ORCID,Zou Wei⁵⁶⁷⁸⁹,Yang Yupei¹⁰¹¹¹²¹³³,Liu Yongbei¹⁰¹¹¹²¹³³,Li Bin¹⁰¹¹¹²¹³³,Qin Yan¹⁰¹¹¹²¹³³,Dang Wenya¹²³⁴,Liu Bin¹²³⁴¹⁰^ORCID,Wang Wei¹⁰¹¹¹²¹³³^ORCID

Affiliation:

1. College of Biology

2. Hunan University

3. Changsha

4. China

5. NHC key laboratory of birth defects research

6. prevention and treatment

7. Hunan Provincial Maternal and Child Health Care Hospital

8. Changsha 410008

9. PR China

10. TCM and Ethnomedicine Innovation & Development International Laboratory

11. Innovative Material Medical Research Institute

12. School of Pharmacy

13. Hunan University of Chinese Medicine

Abstract

T4 polynucleotide kinase (PNK) is the primary member of the 5′-kinase family that can transfer the γ-phosphate residue of ATP to the 5′-hydroxyl group of oligonucleotides.

Funder

Natural Science Foundation of Hunan Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AN/C8AN02147C

Reference46 articles.

1. Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme

2. XRCC1 Stimulates Human Polynucleotide Kinase Activity at Damaged DNA Termini and Accelerates DNA Single-Strand Break Repair

3. Nitrosourea-induced DNA single-strand breaks

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