Multiplexed molecular imaging with surface enhanced resonance Raman scattering nanoprobes reveals immunotherapy response in mice via multichannel image segmentation

Author:

Andreou Chrysafis123ORCID,Plakas Konstantinos4,Berisha Naxhije15,Gigoux Mathieu6,Rosch Lauren E.4,Mirsafavi Rustin1,Oseledchyk Anton1,Pal Suchetan1ORCID,Zamarin Dmitriy6,Merghoub Taha6,Detty Michael R.4,Kircher Moritz F.78

Affiliation:

1. Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA

2. Center for Molecular Imaging and Nanotechnology (CMINT), Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA

3. Department of Electrical and Computer Engineering, University of Cyprus, Nicosia, 1678 Nicosia, Cyprus

4. Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000, USA

5. Department of Chemistry, The Graduate Center of the City University of New York, New York, NY 10016, USA

6. Department of Immunology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA

7. Department of Imaging, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02215, USA

8. Department of Radiology, Brigham & Women's Hospital and Harvard Medical School, Boston, MA 02215, USA

Abstract

Multiplexed imaging of 8 molecular targets with SERS nanoprobes in mice reveals spatially heterogeneous tumor responses to immunotherapy. Multichannel image segmentation enables classification of the tumor regions into responders and naïve.

Funder

Pershing Square Sohn Cancer Research Alliance

Memorial Sloan-Kettering Cancer Center

Damon Runyon Cancer Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

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