A novel dual-release scaffold for fluorescent labels improves cyclic immunofluorescence-Reference-Cited by-同舟云学术

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A novel dual-release scaffold for fluorescent labels improves cyclic immunofluorescence

Published:2024 Issue:7 Volume:5 Page:684-690
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Reiber Thorge¹²,Dose Christian¹,Yushchenko Dmytro A.¹^ORCID

Affiliation:

1. Department of Chemical Biology, Miltenyi Biotec GmbH, Friedrich-Ebert Straße 68, Bergisch Gladbach 51429, Germany

2. Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, Berlin 12489, Germany

Abstract

Cyclic immunofluorescence is a powerful method to generate high-content imaging datasets for investigating cell biology and developing therapies. A dual-release mechanism facilitates fast and efficient cleavage of fluorophores from target antigens.

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CB/D4CB00007B

Reference30 articles.

1. S.Joshi and D.Yu , in Basic Science Methods for Clinical Researchers , ed., M. Jalali , F. Y. L. Saldanha and M. Jalali , Academic Press , Boston , 2017 , pp. 135–150

2. Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

3. Brightness of fluorescent organic nanomaterials

4. Caveat fluorophore: an insiders’ guide to small-molecule fluorescent labels

5. Rational design of small molecule fluorescent probes for biological applications

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