Synthesis of 13C/19F/2H labeled indoles for use as tryptophan precursors for protein NMR spectroscopy-Reference-Cited by-同舟云学术

Synthesis of 13C/19F/2H labeled indoles for use as tryptophan precursors for protein NMR spectroscopy

Published:2021 Issue:23 Volume:19 Page:5133-5147
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Maleckis Ansis¹²³^ORCID,Herath Iresha D.⁴⁵⁶⁷⁸,Otting Gottfried⁴⁵⁶⁷⁸^ORCID

Affiliation:

1. Latvian Institute of Organic Synthesis

2. Riga

3. Latvia

4. ARC Centre of Excellence for Innovations in Peptide & Protein Science

5. Research School of Chemistry

6. Australian National University

7. Canberra

8. Australia

Abstract

Cell-free protein synthesis using ¹⁹F–¹³C and ¹H–¹³C spin pair containing indoles as tryptophan precursors enables site-specific labeling of proteins for ¹⁹F NMR and TROSY NMR studies.

Funder

Australian Research Council

European Regional Development Fund

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/OB/D1OB00611H

Reference74 articles.

1. NMR Studies of Structure and Function of Biological Macromolecules (Nobel Lecture)

2. NMR-Based Methods for Protein Analysis

3. Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research

4. Protein Structure Determination in Living Cells

5. An introduction to NMR-based approaches for measuring protein dynamics

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