Functionalized extracellular nanovesicles as advanced CRISPR delivery systems

Author:

Wang Siqing1,Kong Huimin1,Zhuo Chenya1,Liu Li2,Lv Shixian3,Cheng Du4ORCID,Lao Yeh-Hsing5,Tao Yu1ORCID,Li Mingqiang16ORCID

Affiliation:

1. Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China

2. Department of Gynecology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518000, China

3. School of Materials Science and Engineering, Peking University, Beijing, 100871, China

4. PCFM Lab of Ministry of Education, School of Material Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China

5. Department of Pharmaceutical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA

6. Guangdong Provincial Key Laboratory of Liver Disease Research, Guangzhou 510630, China

Abstract

This review highlights the advances of functional extracellular nanovesicles in CRISPR delivery, including their cellular entry pathways, functionalization techniques, and the challenges and strategies pivotal for clinical translation.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Natural Science Foundation of Guangdong Province

Guangzhou Municipal Science and Technology Project

Guangdong Provincial Pearl River Talents Program

China Primary Health Care Foundation

Publisher

Royal Society of Chemistry (RSC)

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