Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins-Reference-Cited by-同舟云学术

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Published:2019 Issue:20 Volume:9 Page:11038-11045
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Hernandez-Leon Sergio G.¹²³^ORCID,Sarabia-Sainz Jose A.⁴⁵²³^ORCID,Ramos-Clamont Montfort Gabriela¹²³^ORCID,Huerta-Ocampo José Ángel⁶²³^ORCID,Guzman-Partida Ana M.¹²³^ORCID,Robles-Burgueño Maria del Refugio¹²³,Burgara-Estrella Alexel J.⁴⁵²³^ORCID,Vazquez-Moreno Luz¹²³

Affiliation:

1. Centro de Investigación en Alimentación y Desarrollo A.C. Carretera Gustavo Enrique Astiazarán Rosas

2. Hermosillo

3. Mexico

4. Universidad de Sonora. Blvd. Luis Encinas y Rosales S/N

5. Col. Centro

6. CONACYT-Centro de Investigación en Alimentación y Desarrollo A.C. Carretera Gustavo Enrique Astiazarán Rosas

Abstract

Silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni²⁺ ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material.

Funder

Consejo Nacional de Ciencia y Tecnología

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/RA/C9RA01144G

Reference42 articles.

1. Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

2. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

3. Selective binding and magnetic separation of histidine-tagged proteins using Fe3O4/Cu-apatite nanoparticles