High enzymatic activity preservation of malate dehydrogenase immobilized in a Langmuir–Blodgett film and its electrochemical biosensor application for malic acid detection
Author:
Affiliation:
1. Department of Medical Services and Techniques
2. Health Services Vocational School
3. Iğdır University
4. Iğdır
5. Turkey
6. Department of Chemistry
7. Faculty of Sciences
8. Atatürk University
9. Erzurum
Abstract
Malate dehydrogenase was immobilized on a cation octadecylamine monolayer and transferred onto an indium tin oxide coated glass substrate. The enzyme immobilized electrode was then used to electrochemically sense malic acid in the range of 2.2–50 mM.
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemical Engineering,General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2016/RA/C6RA17465E
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