Immunocapture sample clean-up in determination of low abundant protein biomarkers – a feasibility study of peptide capture by anti-protein antibodies-Reference-Cited by-同舟云学术

Immunocapture sample clean-up in determination of low abundant protein biomarkers – a feasibility study of peptide capture by anti-protein antibodies

Published:2019 Issue:60 Volume:9 Page:34902-34911
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Levernæs Maren C. S.¹²³⁴,Farhat Bassem¹²³⁴,Oulie Inger¹²³⁴,Abdullah Sazan S.¹²³⁴,Paus Elisabeth⁵⁶⁷⁴,Reubsaet Léon¹²³⁴^ORCID,Halvorsen Trine G.¹²³⁴^ORCID

Affiliation:

1. Department Pharmacy

2. University of Oslo

3. 0316 Oslo

4. Norway

5. Department of Medical Biochemistry

6. Norwegian Radium Hospital

7. Oslo University Hospital

Abstract

Targeted protein biomarker determination by immunocapture LC-MS/MS: comparison of peptide and protein capture using anti-protein antibodies.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/RA/C9RA05071J

Reference31 articles.

1. Coupling immunoaffinity techniques with MS for quantitative analysis of low-abundance protein biomarkers

2. Techniques for quantitative LC–MS/MS analysis of protein therapeutics: advances in enzyme digestion and immunocapture

3. Evaluation of Large Scale Quantitative Proteomic Assay Development Using Peptide Affinity-based Mass Spectrometry

4. Antibodies as means for selective mass spectrometry

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