Conformational transition in SPR experiments: impact of spacer length, immobilization mode and aptamer density on signal sign and amplitude-Reference-Cited by-同舟云学术

Conformational transition in SPR experiments: impact of spacer length, immobilization mode and aptamer density on signal sign and amplitude

Published:2022 Issue:19 Volume:147 Page:4197-4205
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Pons Marina¹,Perenon Marine¹,Bonnet Hugues¹,Gillon Emilie²,Vallée Celio¹,Coche-Guérente Liliane¹^ORCID,Defrancq Eric¹^ORCID,Spinelli Nicolas¹^ORCID,Van der Heyden Angeline¹,Dejeu Jérôme¹³^ORCID

Affiliation:

1. Univ. Grenoble Alpes, CNRS, DCM UMR-5250, F-38000 Grenoble, France

2. Univ. Grenoble Alpes, CERMAV-CNRS, 601 rue de la chimie, F-38610 Gières, France

3. FEMTO-ST Institute, CNRS UMR-6174, Université de Bourgogne Franche-Comté, F-25000 Besançon, France

Abstract

The spacer length and immobilization mode impact the Surface plasmon resonance (SPR) signal and affinity measured for small target/aptamer recognition. The signal could be positive, negative or null explained by refractive index increment deviation.

Funder

Agence Nationale de la Recherche

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D2AN00824F

Reference40 articles.

1. R. B. M.Schasfoort , Handbook of Surface Plasmon Resonance , 2nd edn, 2017 , pp. 001–524

2. Determination of the Refractive Index Increments of Small Molecules for Correction of Surface Plasmon Resonance Data

3. Using Receptor Conformational Change To Detect Low Molecular Weight Analytes by Surface Plasmon Resonance