Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions-Reference-Cited by-同舟云学术

Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions

Published:2015 Issue:22 Volume:140 Page:7613-7621
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Fang Pan¹²³⁴,Liu Mingqi¹²³⁴,Xue Yu⁵²³⁴,Yao Jun¹²³⁴,Zhang Yang¹²³⁴,Shen Huali¹²³⁴⁶,Yang Pengyuan¹²³⁴⁵

Affiliation:

1. Minhang Hospital and Institutes of Biomedical Sciences

2. Fudan University

3. Shanghai

4. P. R. China

5. Department of Chemistry

6. Department of Systems Biology for Medicine and School of Basic Medical Sciences

Abstract

The highlight of this study is the efficient control of nonspecific trypsin cleavages in shotgun proteomics and N-glycoproteomics using optimized experimental conditions, which greatly increased the specificity of trypsin.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AN/C5AN01505G

Reference27 articles.

1. Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues

2. The Implications of Proteolytic Background for Shotgun Proteomics

3. In-depth Characterization of the Cerebrospinal Fluid (CSF) Proteome Displayed Through the CSF Proteome Resource (CSF-PR)

4. Efficient and Specific Trypsin Digestion of Microgram to Nanogram Quantities of Proteins in Organic−Aqueous Solvent Systems

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